產(chǎn)品應(yīng)用
Biological Activity：Measure by its ability to induce cytotoxicity in PK15 cells in the presence of the actinomycin D.The ED50 for this effect is <15 pg/mL.
SDS-PAGE analysis of recombinant swine TNF alpha
Note：重懸液進一步稀釋分裝時，須添加一定量的載體蛋白（0.1%BSA、10%FBS或5%HSA），無血清實驗需求時，可更換為5%海藻糖溶液作為載體.
Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use ！
- 應(yīng)用：細(xì)胞培養(yǎng)
- 生物學(xué)活性：Measure by its ability to induce cytotoxicity in PK15 cells in the presence of the actinomycin D.The ED50 for this effect is <15 pg/mL.
- 內(nèi)毒素水平：<0.1 EU per 1 μg of the protein by the LAL method.
- 產(chǎn)品組成：從含有1X PBS，pH7.4. 溶液中凍干的蛋白質(zhì).
- 產(chǎn)地：臺灣
- 存儲：-20℃凍干粉長期保存；重懸后的溶液可進一步稀釋分裝，置于2-8℃有效期1個月，-20℃至-80℃有效期3個月；避免反復(fù)凍融.
- 來源：Escherichia coli
- 純度：>98% as determined by SDS-PAGE.Ni-NTA chromatography.
- 氨基酸序列：MLRSSSQTSDKPVAHVVANVKAEGQLQWQSGYANALLANGVKLKDNQLVVPTDGLYLIYSQVLFRGQGCPSTNVFLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKDDRLSAEINLPDYLDFAESGQVYFGIIAL with polyhistidine tag at the C-terminus.
- 復(fù)溶：推薦使用無菌水重懸凍干粉，溶液濃度不低于100μg/mL，并靜置至少20min以充分溶解.
- 運輸：常溫/藍冰
