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Recombinant Mouse TNF alpha (Tumor necrosis factor alpha)

Recombinant TNF alpha, Mouse

產(chǎn)品品牌 產(chǎn)品編號(hào) 規(guī)格 單價(jià) 貨期 mucode 選擇
Chamot CM038-5MP 5 μg 600.00 現(xiàn)貨
Chamot CM038-20MP 20 μg 1500.00 現(xiàn)貨
Chamot CM038-100MP 100 μg 4500.00 現(xiàn)貨
Chamot CM038-500MP 500 μg 13000.00 2周左右
Chamot CM038-1000MP 1 mg 20000.00 2周左右
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產(chǎn)品應(yīng)用

Biological Activity：Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is <40 pg/mL. The specific activity of recombinant mouse TNF alpha is approximately >2.5x 107 IU/mg.

 

SDS-PAGE analysis of recombinant mouse TNF alpha

 

 

 

Note：重懸液進(jìn)一步稀釋分裝時(shí)，須添加一定量的載體蛋白（0.1%BSA、10%FBS或5%HSA），無(wú)血清實(shí)驗(yàn)需求時(shí)，可更換為5%海藻糖溶液作為載體.

 

Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use ！

 

 

應(yīng)用：細(xì)胞培養(yǎng)
生物學(xué)活性：Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is <40 pg/mL. The specific activity of recombinant mouse TNF alpha is approximately >2.5x 107 IU/mg.
內(nèi)毒素水平：<0.1 EU per 1 μg of the protein by the LAL method.
產(chǎn)品組成：N/A
產(chǎn)地：臺(tái)灣
存儲(chǔ)：-20℃凍干粉長(zhǎng)期保存；重懸后的溶液可進(jìn)一步稀釋分裝，置于2-8℃有效期1個(gè)月，-20℃至-80℃有效期3個(gè)月；避免反復(fù)凍融.
來(lái)源：Escherichia coli
純度：>98% as determined by SDS-PAGE. Ni-NTA chromatography.
氨基酸序列：MLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEGDQLSAEVNLPKYLDFAESGQVYFGVIAL with polyhistidine tag at the C-terminus
復(fù)溶：推薦使用無(wú)菌水重懸凍干粉，溶液濃度不低于100μg/mL，并靜置至少20min以充分溶解.
運(yùn)輸：常溫/藍(lán)冰

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